New 2023 Cytiva Biacore 1K

Biacore™ 1 series is a one needle, six flow-cell platform that transforms interaction analysis using surface plasmon resonance (SPR) — making it simpler and faster to use without compromising data quality.

ParameterBiacore™ 1K SPR systemAnalysis temperature25°C to 37°C Analysis time per cycleTypically 2 to 15 min ApplicationKinetic characterization (Biacore Single Cycle Kinetics (SCK)™ and Biacore Multi Cycle Kinetics (MCK)™), affinity characterization, concentration and potency analysis, epitope binning, selectivity studies, kinetic screening single concentration, LMW interaction analysis, fragment screening (Clean screen, Binding level screen, Affinity screen), buffer scouting, competition assay, multi-complex analysis Association rate constant (k_a)Proteins: up to 3 × 10⁹ M^-1 s^-1 Automation60 h unattended operation Baseline noiseTypically < 0.03 RU (RMS) Capacity1 × 96- or 384-well microplate, normal, and deep-well 1 × reagent rack with 21-43 positions compatible with 0.7-4.4 mL vials Depth666 mm Detection technologySurface plasmon resonance (SPR) biosensor Dissociation rate constant (k_d)10^-6 to 1 s^-1 Flow cell volume60 nL Flow rate1 to 100 μL/min Height725 mm In-line reference subtractionAutomatic IncludesBiacore™ 1K instrument, 2 licenses of Biacore™ Insight Software Injection volume1 to 400 μL Mains requirementProcessing unit: Autorange voltage 100 to 240 V~, frequency 50/60 Hz  Molecular weight detectionNo lower limit for organic molecules Number of flow cells6 in 1 channel Power consumptionProcessing unit: max. 350 VA Refractive index1.33 to 1.39 Sample concentration min.≥ 1 picomolar (pM) Sample typeSmall-molecule drug candidates to high-molecular weight proteins (also DNA, RNA, polysaccharides, lipids, cells, and viruses) in various sample environments (e.g., in DMSO-containing buffers, plasma, and serum) Sample volume min.Injection volume plus 20 to 40 μL (application dependent)